Acknowledgements We thank Patricia K. Lankford for Western-blot technical support and the helpful comments from anonymous reviewers for the revision of this manuscript. find more This work is sponsored by the Laboratory Directed Research and Development Program of Oak Ridge National Laboratory (ORNL), managed by UT-Battelle, LLC for the U. S. Department of Energy under Contract No. DE-AC05-00OR22725. The BioEnergy Science Center is a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. Electronic supplementary material Additional file 1: PPT The comparison of Z. mobilis Hfq protein with homologues from
other species. Domain and motif sites of Z. mobilis Hfq (A), E. coli Hfq (B), S. cerevisiae Sm B (D), and S. cerevisiae Lsm1 (E) proteins based on NCBI BlastP result as well as the alignment
for some bacterial BTK inhibitor hfq homologues (C) using ClustalW 2 http://www.ebi.ac.uk/Tools/clustalw2/index.html. Residues that are identical across the species are indicated by “”*”", and residues that are not identical but conserved in function across the species are indicated by “”:”". (PPT ) Additional file 2: PPT Map of plasmid vector pBBR3DEST42. The vector map of pBBR3DEST42 plasmid constructed to analyze gene over-expressing and complementation. Tc(R): Tetracycline resistance gene tet; Cm: chloramphenicol resistance gene cat. attR1 and attR2 are recombination sites allowing recombinational cloning of the gene of interest from an entry clone; ccdB is ccdB gene allowing negative selection of expression clones. (PPT ) Additional file 3: PPT Lsm proteins in S. cerevisiae are involved in multiple inhibitor
tolerance. S. cerevisiae strains were grown in CM with 2% glucose (CM + glucose) for wild-type BY4741 and the deletion mutants, CM with 2% glucose and 2% DMXAA in vitro galactose minus uracil (CM + glucose + 2% PJ34 HCl galactose) for GST overexpression strains. Five-μL culture was then transferred into 250-μL CM broth in the Bioscreen plate. The growth differences of different deletion mutant strains were monitored by Bioscreen (Growth Curves USA, NJ) in CM + glucose at pH 5.5 (A), CM + glucose with 305 mM NaCl, pH 5.5 (B), 305 mM NaAc, pH 5.5 (C), 305 mM NH4OAc, pH 5.5 (D), and 305 mM KAc, pH 5.5 (E), 0.75 g/L vanillin, pH 5.5 (F), 1.5 g/L furfural, pH 5.5 (G), and 1.5 g/L HMF, pH 5.5 (H). The growth differences of different GST-over-expressing strains were monitored by Bioscreen (Growth Curves USA, NJ) in CM + glucose + 2% galactose at pH 5.5 (I), CM + glucose + 2% galactose with 305 mM NaCl, pH 5.5 (J), 305 mM NaAc, pH 5.5 (K), 305 mM NH4OAc, pH 5.5 (L), 305 mM KAc, pH 5.5 (M), 0.75 g/L vanillin, pH 5.5 (N), 1.5 g/L furfural, pH 5.5 (O), and 1.5 g/L HMF, pH 5.5 (P). Strains included in this study are listed in table 1. This experiment has been repeated at least three times with similar result. (PPT ) References 1.