Bat infection was screened with specific SBI-0206965 clinical trial molecular markers for each pathogen, as described in the Methods section. Table 2 displays the number of infected bats with H. capsulatum

in relation to the total number of each bat species sampled at different localities from the monitored Latin American countries. Detection of Pneumocystis spp. infection in the bat lung samples Of the 122 lungs that were molecularly screened for Pneumocystis spp., 51 bats generated sequences for one or both of the Pneumocystis molecular markers assayed. From these sequences, seven matched the mtLSUrRNA locus and another seven matched the mtSSUrRNA locus, while 37 sequences were generated at both loci. Pneumocystis spp. infection alone was found only in eight bats, corresponding to 6.6% (95% CI = 2.25-10.85%) of the total bats studied (Figure 1). Table 2 displays the number of infected bats with Pneumocystis spp. in relation to the total number of each

bat species sampled at different localities from the monitored Latin American countries. H. capsulatum and Pneumocystis spp. co-infection in the bat lung samples Of the lung samples from the 122 bats captured in Argentina, French Guyana, and Mexico that were molecularly screened for H. capsulatum and Pneumocystis infections, 43 samples revealed the specific sequences of each learn more pathogen, corresponding to 35.2% (95% CI = 26.8-43.6%) of the samples being co-infected with both pathogens in bats from the three geographical regions studied (Figure 1). Table 3 displays the number of co-infected bats with both pathogens in relation to the total number of each bat species sampled at different localities from the monitored Latin American countries. Table 3 Species, numbers, and geographical origins of the bats co-infected with H. capsulatum and Pneumocystis spp. Species Geographical origins/localities   Argentina (n = 21) French

Guyana (n = 13) Mexico (n = 88) Number of co-infected bats   TUC CBA Kourou CS MN GR HG MS NL (Total samples per species) Co-infection (total samples) A. hirsutus               3 (5)   3 (5) C. buy PF-01367338 perspicillata     0 (1)             0 over (1) G. soricina     1 (12)     3 (4)       4 (16) N. stramineus           1 (8)       1 (8) P. davyi           0 (1)       0 (1) P. parnellii           0 (2) 0 (1)     0 (3) M. megalophylla           0 (2)     0 (1) 0 (3) T. brasiliensis 8 (16) 0 (5)   2 (8) 2 (8)   3 (20)   19 (27) 34 (84) M. californicus                 1 (1) 1 (1) Number of co-infected bats (Total samples per locality) 8 (16) 0 (5) 1 (13) 2 (8) 2 (8) 4 (17) 3 (21) 3 (5) 20 (29) 43 (122) Abbreviations: TUC = Tucumán; CBA = Córdoba; CS = Chiapas; MN = Michoacán; GR = Guerrero; HG = Hidalgo; MS = Morelos; NL = Nuevo León. Finally, of the total number of bat lungs sampled, 106 (86.8%, 95% CI = 80.92-92.68%) were found to be infected with one or both pathogens, whereas 16 (13.1%, 95% CI = 7.22-18.

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