When upper 2.5 % value of FPG is calculated by
the equation as geometric mean multiplied by the square value of geometric standard deviation, it becomes 146 mg/dl. The HOMA-IR should be handled with caution in their study. Although they did not use HOMA-IR as a dependent variable of logistic regression analysis as a main outcome, the basic information on statistics and application of biological indicator should be clarified to keep validation of their study. References 1. Iki M, Tamaki J, Fujita Y, Kouda K, Yura A, Kadowaki E, Sato Y, Moon JS, Tomioka SN-38 price K, Okamoto N, Kurumatani N (2012) Serum undercarboxylated osteocalcin levels are inversely associated with glycemic status and insulin resistance in an elderly Japanese male population: Fujiwara-kyo Osteoporosis Risk in Men (FORMEN) study. Osteoporos Int 23:761–770. doi:10.1007/s00198-011-1600-7 PubMedCrossRef 2. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC (1985) Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 28:412–419PubMedCrossRef 3. Levy JC, Matthews DR, Hermans MP (1998) Correct homeostasis model assessment (HOMA) evaluation uses the computer program. Diabetes Care 21:2191–2192PubMedCrossRef 4. Wallace EPZ015938 cell line TM, Levy JC,
Matthews DR (2004) Use and abuse of HOMA modeling. Diabetes Care 27:1487–1495PubMedCrossRef Mirabegron 5. Nolan JJ, Farch K (2012) Estimating insulin sensitivity and beta cell function: perspectives from the modern Foretinib solubility dmso pandemics of obesity and type 2 diabetes. Diabetologia 55:2863–2867PubMedCrossRef”
“Dear Editor, We appreciate Dr. Kawada for giving us two queries on our article  concerning representativeness of the participants of our study for the general male population since their fasting plasma glucose (FPG) and serum lipid levels did not distribute normally, and applicability of the homeostasis model assessment of insulin resistance
(HOMA-IR) to the participants including those with hyperglycemia. For the first query, FPG and serum lipid levels of our study participants distributed log normally rather than normally indicated by the Shapiro–Wilk statistic which is known to have much greater statistical power than χ 2 test for goodness of fit. Although Dr. Kawada stated that FPG levels distributed normally from his experience, FPG values of men aged 60 years and older randomly selected from the Japanese population in the National Health and Nutrition Survey (NHNS) in 2010  did not distribute normally according to the Shapiro–Wilk statistic (p < 0.0001). Furthermore, the prevalence of diabetes mellitus in our subjects was 17.9 % which was not significantly different from 19.5 % for males aged 60 years and older as reported in NHNS.