No distinct odour noted Conidiation noted after 2–3 days, on sev

No distinct odour noted. Conidiation noted after 2–3 days, on several thick mononematous conidiophores with a gliocladium-like apical penicillus arising from common bases forming micropustules to 0.6 mm diam,

superposed by aerial hyphae. Conidia formed in small numbers in wet or dry heads, remaining colourless; only few heads appearing greenish in the stereo-microscope. Poor conidial yield also noted at other temperatures. Phialides more divergent than on CMD and SNA. On SNA after 72 h 11–14 mm at 15°C, 25–28 mm at 25°C, 16–17 mm at 30°C; mycelium covering the plate after 10–11 days at 25°C. Colony hyaline, thin, circular, dense, not zonate; mycelium radial, scarce on the agar surface; margin wavy. Aerial hyphae scant or lacking. Autolytic excretions and coilings lacking. No diffusing pigment, no distinct odour noted. Chlamydospores noted after 1–3 weeks, infrequent and inconspicuous, mostly terminal, (5–)6–10(–12) × (5–)6–9(–10)

μm, l/w 1.0–1.7 (n = 30), globose or MK-8931 MLN2238 chemical structure pyriform. Conidiation at 25°C noted after 8–9 days, green to black after 2 weeks; first appearing as solitary, simple, mononematous gliocladium-like brushes; later pustules appearing mainly on the distal margin or irregularly distributed, white, becoming dark green to black. Pustules growing to 1 mm diam, with conidia formed abundantly in wet heads to ca 250 μm diam, growing and confluent to 600 μm diam, green, turning black; small and light green when dry. At 15°C similar, development slower, conidiation in small pustules. Conidiation positively correlated with the temperature, most abundant at 30°C. At 30°C conidiation in green, mostly 2–3E4–6, pustules loosely distributed in several ill-defined concentric zones or irregularly distributed. Pustules to 1(–1.5) mm diam, confluent very to 2.5 mm long, with numerous, radially EX 527 cell line arranged, straight gliocladium-like conidiophores and wet conidial heads 80–250 μm diam; with high conidial yield. Development and maturation asynchronous, i.e. numerous fresh, small, light green heads formed above older, large, dark green heads in the same pustule. Branches in pustules (after 12–22 days) loose, often in right angles, giving rise

to additional few or numerous, radially arranged, straight, mononematous gliocladium-like stipes; the latter mostly 100–250 μm long, more rarely to 400 μm, 7–9(–10) μm wide at the base, attenuated upwards to 3–6(–8) μm to the first branching point; appearing verrucose under low magnification due to guttules, in mounts smooth, to somewhat verrucose when old. Each conidiophore with a single penicillus typically 20–35 μm diam and length at the apex, including the phialides; with 1–3 branching levels. First level symmetric or asymmetric, sometimes of only 1, but mostly several steep branches arising from a single point at each level. Branches nearly parallel, 3–5 μm wide, last branches (metulae) often resembling phialides in shape and size, 6–8 × 2.5–3.5(–4.5) μm, often slightly thickened at the apex.

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