We have developed a model, which uses a real-time stable reporting system incorporating our bioluminescent tagged Salmonella enterica serotypes, which can be used to evaluate various pathogenic mitigation strategies. Further, this model may eventually aid in the understanding of how these serotypes are able to survive the processing continuum. We performed this selleck kinase inhibitor experiment to demonstrate the potential value of this model as a screening tool by evaluating the performance of our bioluminescent Salmonella on chicken skin sections at two temperatures in an aqueous environment. We selected S. Mbandaka and S. Montevideo for this skin attachment experiment

based on the consistent bioluminescence expression we observed within these serotypes (Figure 3). Individual aqueous CHIR-99021 in vivo solutions, each containing a Salmonella enterica serotype, were prepared and introduced to chicken skin according to protocol (described below).

Separate plates (24-well) containing replicates of each serotype were placed on a rotating stage at 4°C and 25°C for 2 h. Immediately following this step, bioluminescent imaging was collected after a five minute interval at 37°C for both serotypes and is reported (Figure 4). Bioluminescent monitoring demonstrated the ability to quantify bacteria numbers on chicken skin following cold and warm washes. Our previous work showed washing with 25°C water suppressed the reproduction of Salmonella {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| on chicken skin likely through the physical removal of bacteria [19]. Given that Salmonella is a mesophile, refrigeration temperatures further limit bacterial growth and the bacteria become metabolically static. Bioluminescent values, confirming bacteria numbers, at post-wash (4°C) were not shown to be significantly different compared to pre-wash values for both

serotypes (P ≥ 0.25). HA 1077 Bioluminescent values at post-wash (25°C) were greater compared to pre-wash values but the difference was not shown to be significantly different (P ≥ 0.125). The increase in bioluminescence following the 25°C wash period is due to increased bacteria growth under favorable metabolic conditions (temperature) and nutrients provided by the chicken skin in solution. With our model we were able to quantify a change in bacteria number by monitoring bioluminescence following treatment. Figure 4 Monitoring bacteria number following 25°C and 4°C water washes. Bioluminescence quantified at 37°C before and after water washes at 4°C and 25°C. A) S. Mbandaka. B) S. Montevideo. These results provide evidence that our model may serve as an accurate and efficient means for in-vitro evaluation of the efficacy of pathogen mitigation strategies, i.e. antimicrobial compounds (AMC) and processing parameters, that may be utilized in the poultry processing industry to control Salmonella enterica.