Therefore, the initiator methionine is not the one indicated in the database, and the protein

is 298 amino acids. Surprisingly, there is no obvious Shine-Dalgarno sequence adjacent to the initiator methionine www.selleckchem.com/products/verubecestat-mk-8931.html we identified (Figure 5). Figure 5 Determination of the first methionine of GluQ-RS. The cloning strategy utilized is shown at the top. A fragment from the stop codon of dksA to the end of gluQ-rs gene was amplified from S. flexneri genomic DNA with the primers ATGGQRSF/ATGGQRSR and cloned into a pET15c vector using the restriction sites BamHI and XhoI. Therefore this clone represents the operon, but dksA was replaced with the plasmid encoded fragment pelB. The transcription of this plasmid, named pATGGQRS, is controlled by the T7 promoter and translation is controlled by the Shine-Dalgarno (SD) sequence of pelB, both contained

within the plasmid. The GluQ-RS protein synthesized has a histidine tag (H6) at the C-terminus, facilitating protein purification. The {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| putative ρ-independent terminator is represented by the stem loop symbol upstream of gluQ-rs gene , and the black box in the gene indicates the two possible peptides, depending of which methionine is utilized. Bottom: The SDS –polacrylamide gel electrophoresis showing the supernatant extract (S) and the partially purified protein (Pur) produced by cells carrying the selleck recombinant plasmid. The predominant band indicated by the arrow was excised and subjected to amino terminal sequencing, yielding the following amino acid sequence: T-D-T-Q-Y-I-G-R-F-A-P. This corresponds to the sequence following the second methionine. The size of the molecular markers

(M) are given in kDa. Phenotype of the S. flexneri gluQ-rs mutant To determine the role of GluQ-RS in S. flexneri growth and virulence, a deletion mutant of the gluQ-rs gene was constructed in S. flexneri 2457T. The mutant was compared to the wild type by Biolog phenotype MicroArrays (Biolog, Inc., Almeda, CA). The major difference observed for the mutant was impaired metabolism when grown under osmotic stress conditions (Figure 6). The mutant had a longer lag and reduced growth Oxymatrine compared to the wild type in the presence of increasing concentrations of potassium chloride, sodium sulfate, sodium formate, sodium benzoate, sodium nitrate and sodium nitrite. The phenotype was complemented with the gluQ-rs gene cloned into an expression vector. No differences were observed in the growth or metabolism of these strains when they were incubated in presence of 1% sodium chloride, which was similar to LB (Figure 6 and data not shown). Figure 6 The gluQ-rs mutant is sensitive to growth in high osmolarity. Biolog phenotypic MicroArrays were used to characterize the growth and metabolism of the gluQ-rs mutant and its wild type parent, 2457T. Wild type (black line) transformed with the empty plasmid, 2457T ΔgluQ-rs::kan (red line) transformed with the empty plasmid pCM and S.