Methods: The expression patterns for FAK and PTEN along human tissues, as well as in orthotropic gastric cancer nude mice model were examined. The effect of PTEN or PI3K inhibition and NF-κB inhibitor PDTC on FAK RNA and protein expression. NF-κB DNA binding activity,and

the promoter activity of the FAK gene was analyzed by luciferase-reporter method,electrophoretic mobility shift assay (EMSA)and chromatin immunoprecipitation(ChIP)analysis in human gastric cancer cell lines. Results: PTEN overexpression or knockdown in gastric cancer cells led to the downregulation or upregulation of focal adhesion kinase (FAK), and decreased or increased cell migration and invasion, respectively. Moreover,FAK overexpression Target Selective Inhibitor Library solubility dmso Selleck BAY 57-1293 could rescue the inhibition of cell migration and invasion by PTEN. These results were further confirmed in orthotropic gastric cancer nude mice model. In addition, in human gastric cancer tissues, PTEN protein level was conversely correlated with FAK protein level. Mechanistically, we found

that PTEN inhibited PI3K/NF-κB pathway and inhibited the DNA binding of NF-κB on FAK promoter. Conclusion: Taken together, our data reveal a novel mechanism that PTEN inhibits the growth and invasion of gastric cancer via the downregulation of FAK expression, and suggest that exploiting PTEN/PI3K/NF-kappaB/FAK axis is a promising approach to treat gastric cancer metastasis. Key Word(s): 1. gastric cancer; 2. PTEN; 3. FAK; 4. NF-kB; Presenting Author: 明炯 Additional Authors: 鹏 李, 澍田 Corresponding Author: 明炯 Affiliations: 北京友谊医院; Objective: Background:Esophageal cancer is one of the most common malignant tumor worldwide.each year, which cause 480000 new find more cases and 360000

death, more than half of them occurred in China, so esophageal cancer is a big problem to our country. The incidence of esophageal cancer in China is different from the developed countries,most esophageal carcinoma in China is mainly esophageal squamous cell carcinoma (ESCC), so the study of ESCC is not only related to the public health, it also has the Chinese characteristic in research. Molecular mechanism and etiology of esophageal squamous cell carcinoma has not been fully elucidated. It has been shown smoking is strongly associated with lung cancer, pancreatic cancer, breast cancer, colon cancer, prostate cancer and other malignant tumors, but there is little research in esophageal squamous cell carcinoma. Aims: To ivestigate the role of β-Adrenergic Receptor in Esophageal Squamous Cell Carcinoma caused by tobacco in mouse model. Methods: Methods: (1)Nude mice model: The esophageal squamous cell carcinoma cell line KYS410 was inoculated subcutaneously into nude mice. The nude mice were randomly divided into 6 groups: control group, atenolol group, ICI118551 group, NNK group, NNK+ atenolol group, NNK + ICI118551 group.