Intestinal BMS-354825 clinical trial microbiota has been implicated in the pathogenesis of celiac disease. Methods: In this preliminary study, we explored the differences in bacterial community composition in a patient with celiac disease both before and six months after gluten free diet and compared that with a patient with functional dyspepsia. Total community DNA was extracted from fecal samples and 16S rRNA gene variable region V3 was amplified and sequenced using NGS platform Ion torrent PGMTM. In addition, absolute

quantification of major bacterial genera was done using real time qPCR. Results: The OTU based network analysis showed presence of a distinct gut bacterial community in patients with celiac disease

at base line and six months after GFD and control. A relative decrease in Proteobacteria was observed after six months of GFD in patient with celiac disease. Pathogenic bacteria such as Campylobacter sp. and Haemophilus sp., which were present in treatment naïve state, were not detected FDA approved Drug Library manufacturer after six months of GFD. Furthermore, an increase in the ‘beneficial’ bacteria such as Bifidobacterium was observed six months after GFD. qPCR analysis confirmed the increase in number of Bifidobacterium after GFD. Conclusion: There is a distinct intestinal microbiome in patients with celiac disease both before and after GFD. After GFD, there is a shift in the bacterial community composition towards a healthy gut microbiome. Larger study is required. Key Word(s): 1. Celiac Diesase; 2. Gut Microbiota; 3. India; 4. NGS; Presenting Author: JIN HAIFENG Additional Authors: LV BIN, ZHAO MIAN Corresponding Author: JIN for HAIFENG Affiliations: Zhejiang province hospitol

of TCM Objective: To study the effect and possible mechanism of curcuma wenyujin diterpenoid compound C on lipopolysaccharide-induced (LPS-induced) release of inflammatory factors in gastric cancer cells. Methods: Human gastric cancer SGC-7901 cells were affected by curcuma wenyujin diterpenoid compound C with different concentrations in vitro at different times. The growth inhibition ratio of human gastric cancer SGC-7901 cells was measured by MTT assay, secretion of inflammatory factors IL-1β and IL-2 was detected by ELISA, mRNA transcriptions of the two inflammatory factors were were assessed by Western Blot. Results: Curcuma wenyujin diterpenoid compound C within 10 ng/mL and LPS within 10 ng/mL had no effect on the proliferation of SGC-7901. Diterpenoid compound C significantly inhibited LPS-induced release of inflammatory factor IL-1β and increased the release of inflammatory depressive factor IL-2. No significant difference was found in RT-PCR detection result. Curcuma wenyujin diterpenoid compound C inhibited the expression of P38, JNK and ERK protein in the MAPK pathway.