Increased abundance of ribosomal CB-5083 order proteins is seen under conditions of increased growth rate in all domains of life [27–29]. However, we have found that internalized P. gingivalis maintain viability and replicate slowly within gingival epithelial cells [3]. Thus, an overall increase in protein expression due to increased energy production may be responsible for the increased abundance of translational machinery, more so than growth under these conditions. Table 3 A list of detected proteins, by P. gingivalis PGN number [11], assigned to ribosomal proteins as determined using DAVID. Increased
(32) Unchanged (19) Decreased Levels (1) PGN_0035 PGN_0167 PGN_0640 PGN_0965 PGN_0394 PGN_0188 PGN_0279 PGN_1572 PGN_1589 PGN_0636 PGN_0639 PGN_1647 PGN_1648 PGN_0641 learn more PGN_0964 PGN_1698 PGN_1844 PGN_1088 PGN_1219 PGN_1651 PGN_1852 PGN_1573 PGN_1575 PGN_1853 PGN_1854 PGN_1588 PGN_1590 PGN_1855 PGN_1861 PGN_1832 PGN_1840 PGN_1863 PGN_1868 PGN_1842 PGN_1843 PGN_1872 PGN_1890 PGN_1849 PGN_1850 PGN_1891 PGN_1856 PGN_1857 PGN_1857 PGN_1858 PGN_1860 PGN_1862 PGN_1864 PGN_1865 PGN_1866 PGN_1867 PGN_1869 PGN_1871 Proteins are indicated as increased, decreased or unchanged in abundance for internalized P. gingivalis versus www.selleckchem.com/products/MGCD0103(Mocetinostat).html external
control cells. The totals for each category are given in parentheses. Table 4 A list of detected proteins, by P. gingivalis PGN number [11], assigned to translation initiation, elongation and termination as determined using DAVID. Increased (8) Unchanged (3) Decreased Levels
(0) PGN_0355 PGN_0963 PGN_0313 PGN_1014 PGN_1405 PGN_1578 PGN_1244 PGN_1587 PGN_1846 PGN_1870 PGN_2022 Proteins are listed by ORF number in the same manner as in Table 3. Table 5 G protein-coupled receptor kinase A list of detected proteins, by P. gingivalis PGN number [11], assigned to tRNA synthetases and transferases as determined using DAVID. Increased (16) Unchanged (8) Decreased Levels (3) PGN_0209 PGN_0360 PGN_0137 PGN_0278 PGN_0266 PGN_0278 PGN_0365 PGN_0517 PGN_0281 PGN_0366 PGN_1157 PGN_0543 PGN_0570 PGN_0569 PGN_0981 PGN_0819 PGN_0962 PGN_1711 PGN_1883 PGN_0987 PGN_1218 PGN_1229 PGN_1381 PGN_1805 PGN_1969 PGN_2045 PGN_2060 Proteins are listed by ORF number in the same manner as in Table 3. Transcription machinery Most of the proteins responsible for transcription also showed increased abundance (Table 6, Additional file 1: Table S1). This is consistent with the overall increase in translational machinery as well as the larger number of proteins showing increased versus decreased abundance within gingival epithelial cells. Table 6 A list of detected proteins, by P. gingivalis PGN number [11], assigned to transcription as determined using DAVID.