Briefly, the rats were anesthetized with sodium pentobar bital. The left renal artery was exposed by retroperitoneal flank incision and dissected free of charge with the renal vein and connective tissue. A silver clip using a lumen of 0. 22 mm was positioned close to the artery for partial occlusion, in sham operations, the artery was not clipped. Soon after 6 weeks the systolic blood strain was mea sured working with the tail cuff strategy in aware rats. Only hypertensive rats have been used in the experiments. Animal treatment method At 6 weeks right after the surgery, when hypertension was established, the 2K1C rats had been divided into 5 groups of ten 12 rats, groups 1 sham operated manage rats, groups two an untreated model group, groups three rats treated with captopril, groups 4 rats taken care of with low dose DOT, group five rats handled with higher dose DOT, Rats have been treated for 6 weeks with daily oral administration of the solutions or even the similar volume of car.
Doses of DOT had been selected in ref erence to doses usually used in human selleck inhibitor and doses used in former experiments. All rats had been weighed and their blood strain was measured when a week for four weeks. Measurement of blood strain Systolic blood stress and diastolic blood strain have been measured by the tail cuff approach in awaken rats. Just about every value was the common of 3 consecutive read ings. The arterial systolic and diastolic blood stress was measured on the weekend and continuous measurement was carried out 3 times as the regular blood strain for weeks. Excess weight was measured the moment a week. Measurement of left ventricular hypertrophy and cardiac mass index The heart and three cm of left ventricular hypertrophy were removed.
The cardiac mass index was the ratio with the rat heart weight for the physique excess weight. selleck chemical The left heart bodyweight and left ventricular anterior wall thickness have been also measured. Assessment on the renal function On the last week of your experiment, the animals had been positioned in individual metabolic cages and acclimatized for two consecutive days just before a 24 h urine assortment. Cre atinine and urea have been measured by using a commercial enzyme linked immunosorbent assay as described from the manufacturer. Determination of SOD action and lipid peroxides level Superoxide dismutase exercise and the malondialde hyde level have been established in accordance towards the in structions within the kit. Determination of plasma angiotensin II and endothelin concentration Right after the last measurement of blood strain, the 2K1C rats were fasted for twelve h and anesthetized with sodium pentobarbital. Blood samples had been taken from the abdominal aorta and dealt with as follows, 4 ml were collected on aprotinin and 10% EDTA disodium, then centri fuged at four C, at 3000 r. p. m. for 10 min and the plasma obtained was aliquoted and frozen for biochemical ana lysis.